Inducible gene targeting in mice
A method of gene targeting that allows the inducible inactivation of a target gene in mice is
presented. The method uses an interferon-responsive promoter to control the expression of
Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase β gene
flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly
complete in lymphocytes within a few days, whereas partial deletion was obtained in other
tissues. This method can be used for the inducible inactivation of any other gene in vivo.
presented. The method uses an interferon-responsive promoter to control the expression of
Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase β gene
flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly
complete in lymphocytes within a few days, whereas partial deletion was obtained in other
tissues. This method can be used for the inducible inactivation of any other gene in vivo.
A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase β gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.
AAAS