[HTML][HTML] Activation of the bumetanide-sensitive Na+, K+, 2Cl− cotransporter (NKCC2) is facilitated by Tamm-Horsfall protein in a chloride-sensitive manner
K Mutig, T Kahl, T Saritas, M Godes, P Persson… - Journal of Biological …, 2011 - ASBMB
Active transport of NaCl across thick ascending limb (TAL) epithelium is accomplished by
Na+, K+, 2Cl− cotransporter (NKCC2). The activity of NKCC2 is determined by vasopressin
(AVP) or intracellular chloride concentration and includes its amino-terminal
phosphorylation. Co-expressed Tamm-Horsfall protein (THP) has been proposed to interact
with NKCC2. We hypothesized that THP modulates NKCC2 activity in TAL. THP-deficient
mice (THP−/−) showed an increased abundance of intracellular NKCC2 located in subapical …
Na+, K+, 2Cl− cotransporter (NKCC2). The activity of NKCC2 is determined by vasopressin
(AVP) or intracellular chloride concentration and includes its amino-terminal
phosphorylation. Co-expressed Tamm-Horsfall protein (THP) has been proposed to interact
with NKCC2. We hypothesized that THP modulates NKCC2 activity in TAL. THP-deficient
mice (THP−/−) showed an increased abundance of intracellular NKCC2 located in subapical …