Methyl CpG binding protein 2 deficiency enhances expression of inflammatory cytokines by sustaining NF-κB signaling in myeloid derived cells
CM O'Driscoll, MP Lima, WE Kaufmann… - Journal of …, 2015 - Elsevier
Knocking down methyl CpG binding protein 2 (MeCP2) enhances NF-κB activation in
human peripheral blood mononuclear cells (PBMC). In this study, we examined whether this
caused the expression of cytokines to be elevated. Increased levels of TNFα, IL-6, and IL-3
mRNAs were observed in human PBMC made MeCP2 deficient with a lentiviral shRNA
MeCP2 vector and in splenocytes from MeCP2-null mice. TNFα neutralizing antibody
attenuated expression of IL-6 and TNFα but did not affect expression of IL-3 …
human peripheral blood mononuclear cells (PBMC). In this study, we examined whether this
caused the expression of cytokines to be elevated. Increased levels of TNFα, IL-6, and IL-3
mRNAs were observed in human PBMC made MeCP2 deficient with a lentiviral shRNA
MeCP2 vector and in splenocytes from MeCP2-null mice. TNFα neutralizing antibody
attenuated expression of IL-6 and TNFα but did not affect expression of IL-3 …