Spatiotemporally controlled induction of autophagy-mediated lysosome turnover

YH Hung, LMW Chen, JY Yang… - Nature communications, 2013 - nature.com
YH Hung, LMW Chen, JY Yang, W Yuan Yang
Nature communications, 2013nature.com
Lysosomes are the major degradative compartments within cells, harbouring a wide variety
of hydrolytic enzymes within their lumen. Release of lysosomal hydrolases from lysosomes
into the cell cytoplasm results in cell death. Here we report that damaged lysosomes
undergo autophagic turnover. Using a light-induced lysosome impairing scheme that can be
controlled spatially and temporally within a cell, we show that damaged lysosomes are
selectively ubiquitinated, recruit autophagic proteins and are eventually incorporated into …
Abstract
Lysosomes are the major degradative compartments within cells, harbouring a wide variety of hydrolytic enzymes within their lumen. Release of lysosomal hydrolases from lysosomes into the cell cytoplasm results in cell death. Here we report that damaged lysosomes undergo autophagic turnover. Using a light-induced lysosome impairing scheme that can be controlled spatially and temporally within a cell, we show that damaged lysosomes are selectively ubiquitinated, recruit autophagic proteins and are eventually incorporated into autolysosomes for degradation. We propose that autophagic removal of lysosomes, which we term lysophagy, is a surveillance mechanism that alleviates cells from the adverse effects of lysosomal damage. We envision our method to induce lysosomal damage will enable detailed molecular studies of the lysophagy pathway in the future.
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