A novel route for F-box protein-mediated ubiquitination links CHIP to glycoprotein quality control
RF Nelson, KA Glenn, VM Miller, H Wen… - Journal of Biological …, 2006 - jbc.org
In SCF (Skp1/Cullin/F-box protein) ubiquitin ligases, substrate specificity is conferred by a
diverse array of F-box proteins. Only in fully assembled SCF complexes, it is believed, can
substrates bound to F-box proteins become ubiquitinated. Here we show that Fbx2, a brain-
enriched F-box protein implicated in the ubiquitination of glycoproteins discarded from the
endoplasmic reticulum, binds the co-chaperone/ubiquitin ligase CHIP (C terminus of Hsc-70-
interacting protein) through a unique N-terminal PEST domain in Fbx2. CHIP facilitates the …
diverse array of F-box proteins. Only in fully assembled SCF complexes, it is believed, can
substrates bound to F-box proteins become ubiquitinated. Here we show that Fbx2, a brain-
enriched F-box protein implicated in the ubiquitination of glycoproteins discarded from the
endoplasmic reticulum, binds the co-chaperone/ubiquitin ligase CHIP (C terminus of Hsc-70-
interacting protein) through a unique N-terminal PEST domain in Fbx2. CHIP facilitates the …