Multiple surface regions on the Niemann-Pick C2 protein facilitate intracellular cholesterol transport
LA McCauliff, Z Xu, R Li, S Kodukula, DC Ko… - Journal of Biological …, 2015 - jbc.org
The cholesterol storage disorder Niemann-Pick type C (NPC) disease is caused by defects
in either of two late endosomal/lysosomal proteins, NPC1 and NPC2. NPC2 is a 16-kDa
soluble protein that binds cholesterol in a 1: 1 stoichiometry and can transfer cholesterol
between membranes by a mechanism that involves protein-membrane interactions. To
examine the structural basis of NPC2 function in cholesterol trafficking, a series of point
mutations were generated across the surface of the protein. Several NPC2 mutants …
in either of two late endosomal/lysosomal proteins, NPC1 and NPC2. NPC2 is a 16-kDa
soluble protein that binds cholesterol in a 1: 1 stoichiometry and can transfer cholesterol
between membranes by a mechanism that involves protein-membrane interactions. To
examine the structural basis of NPC2 function in cholesterol trafficking, a series of point
mutations were generated across the surface of the protein. Several NPC2 mutants …
