Characterization of genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells …
The clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated
(Cas) 9 nuclease system has provided a powerful tool for genome engineering. Double
strand breaks may trigger nonhomologous end joining repair, leading to frameshift
mutations, or homology-directed repair using an extrachromosomal template. Alternatively,
genomic deletions may be produced by a pair of double strand breaks. The efficiency of
CRISPR/Cas9-mediated genomic deletions has not been systematically explored. Here, we …
(Cas) 9 nuclease system has provided a powerful tool for genome engineering. Double
strand breaks may trigger nonhomologous end joining repair, leading to frameshift
mutations, or homology-directed repair using an extrachromosomal template. Alternatively,
genomic deletions may be produced by a pair of double strand breaks. The efficiency of
CRISPR/Cas9-mediated genomic deletions has not been systematically explored. Here, we …
