Purine nucleoside phosphorylase: a new marker for free oxygen radical injury to the endothelial cell
PN Rao, TR Walsh, L Makowka, RS Rubin, T Weber… - Hepatology, 1990 - journals.lww.com
The effect of ischemia and reperfusion on purine nucleoside phosphorylase was studied in
an isolated perfused rat liver model. This enzyme is localized primarily in the cytoplasm of
the endothelial and Kupffer cells; some activity is associated with the parenchymal cells.
Levels of this enzyme accurately predicted the extent of ischemia and reperfusion damage
to the microvascular endothelial cell of the liver. Livers from Lewis rats were subjected to 30,
45 and 60 min of warm (37 C) no flow ischemia that was followed by a standard reperfusion …
an isolated perfused rat liver model. This enzyme is localized primarily in the cytoplasm of
the endothelial and Kupffer cells; some activity is associated with the parenchymal cells.
Levels of this enzyme accurately predicted the extent of ischemia and reperfusion damage
to the microvascular endothelial cell of the liver. Livers from Lewis rats were subjected to 30,
45 and 60 min of warm (37 C) no flow ischemia that was followed by a standard reperfusion …