A new model of visceral pain and referred hyperalgesia in the mouse
The generation of transgenic mice that lack or overexpress genes relevant to pain is
becoming increasing common. However, only one visceral pain model, the writhing test, is
widely used in mice. Here we describe a novel model, chemical stimulation of the colon,
which we have developed in mice. Mice of either sex were injected iv with 30 mg/kg Evan's
Blue for subsequent determination of plasma extravasation. For behavioural testing, they
were placed on a raised grid and 50 μl of saline, mustard oil (0.25–2.5%) or capsaicin (0.03 …
becoming increasing common. However, only one visceral pain model, the writhing test, is
widely used in mice. Here we describe a novel model, chemical stimulation of the colon,
which we have developed in mice. Mice of either sex were injected iv with 30 mg/kg Evan's
Blue for subsequent determination of plasma extravasation. For behavioural testing, they
were placed on a raised grid and 50 μl of saline, mustard oil (0.25–2.5%) or capsaicin (0.03 …
