Assay for improved detection of antigen-specific immune cells from extrasanguinous fluids.
D Schub, M Fousse, J Elsäßer… - European Journal of …, 2018 - europepmc.org
D Schub, M Fousse, J Elsäßer, K Faßbender, U Sester, T Schmidt, M Sester
European Journal of Immunology, 2018•europepmc.orgIn this approach, pre-stained cells from extrasanguinous fluids (ESFs) are stimulated in the
presence of blood from the same individual. Thus, blood-derived antigen-presenting cells
enable stimulation of both ESF-and blood T cells. Pre-staining allows distinction of T cells
from ESF and blood, and simultaneous analysis of antigen-specific T cells in both
compartments.
presence of blood from the same individual. Thus, blood-derived antigen-presenting cells
enable stimulation of both ESF-and blood T cells. Pre-staining allows distinction of T cells
from ESF and blood, and simultaneous analysis of antigen-specific T cells in both
compartments.
In this approach, pre-stained cells from extrasanguinous fluids (ESFs) are stimulated in the presence of blood from the same individual. Thus, blood-derived antigen-presenting cells enable stimulation of both ESF-and blood T cells. Pre-staining allows distinction of T cells from ESF and blood, and simultaneous analysis of antigen-specific T cells in both compartments.
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