High throughput measurement of extracellular DNA release and quantitative NET formation in human neutrophils in vitro

P Sil, D Yoo, M Floyd, A Gingerich, B Rada - JoVE (Journal of Visualized …, 2016 - jove.com
Neutrophil granulocytes are the most abundant leukocytes in the human blood. Neutrophils
are the first to arrive at the site of infection. Neutrophils developed several antimicrobial
mechanisms including phagocytosis, degranulation and formation of neutrophil extracellular
traps (NETs). NETs consist of a DNA scaffold decorated with histones and several granule
markers including myeloperoxidase (MPO) and human neutrophil elastase (HNE). NET
release is an active process involving characteristic morphological changes of neutrophils …