Assays for the assessment of neutralizing antibody activities against Severe Acute Respiratory Syndrome (SARS) associated coronavirus (SCV)

S Wang, P Sakhatskyy, THW Chou, S Lu - Journal of immunological …, 2005 - Elsevier
Accurate assessment of neutralizing antibody activities is important either for patients
infected with Severe Acute Respiratory Syndrome (SARS) or for animals and volunteers
immunized with the experimental vaccines against the SARS associated coronavirus (SCV).
However, the current assay based on the cytopathic effect (CPE) which has been frequently
cited in literature has several limitations. The CPE assay relies on the visual observation on
the damage of SCV infected target cells under a microscope. It is subjected to observer …