Measurement of the efficacy of vaccines and antimicrobial therapy against infection with Toxoplasma gondii
MJ Kirisits, E Mui, R McLeod - International journal for parasitology, 2000 - Elsevier
To facilitate studies of vaccines and antimicrobial agents effective against Toxoplasma
gondii infection, an assay system was developed to semi-quantitate parasitaemia using
PCR amplification of T. gondii DNA obtained from the blood of mice infected with the
parasite. A competitive internal standard DNA fragment of the B1 gene of T. gondii was
generated and used in PCR so that the amplified product could be semi-quantitated and
false negative results could be avoided. The PCR assay system was used to analyse the …
gondii infection, an assay system was developed to semi-quantitate parasitaemia using
PCR amplification of T. gondii DNA obtained from the blood of mice infected with the
parasite. A competitive internal standard DNA fragment of the B1 gene of T. gondii was
generated and used in PCR so that the amplified product could be semi-quantitated and
false negative results could be avoided. The PCR assay system was used to analyse the …