Isolation, characterization, and high throughput extracellular flux analysis of mouse primary renal tubular epithelial cells
Mitochondrial dysfunction in the renal tubular epithelial cells (TECs) can lead to renal
fibrosis, a major cause of chronic kidney disease (CKD). Therefore, assessing mitochondrial
function in primary TECs may provide valuable insight into the bioenergetic status of the
cells, providing insight into the pathophysiology of CKD. While there are a number of
complex protocols available for the isolation and purification of proximal tubules in different
species, the field lacks a cost-effective method optimized for tubular cell isolation without the …
fibrosis, a major cause of chronic kidney disease (CKD). Therefore, assessing mitochondrial
function in primary TECs may provide valuable insight into the bioenergetic status of the
cells, providing insight into the pathophysiology of CKD. While there are a number of
complex protocols available for the isolation and purification of proximal tubules in different
species, the field lacks a cost-effective method optimized for tubular cell isolation without the …