[HTML][HTML] Nitric-oxide synthase forms N-NO-pterin and S-NO-cys: implications for activity, allostery, and regulation
RJ Rosenfeld, J Bonaventura, BR Szymczyna… - Journal of biological …, 2010 - ASBMB
Inducible nitric-oxide synthase (iNOS) produces biologically stressful levels of nitric oxide
(NO) as a potent mediator of cellular cytotoxicity or signaling. Yet, how this nitrosative stress
affects iNOS function in vivo is poorly understood. Here we define two specific non-heme
iNOS nitrosation sites discovered by combining UV-visible spectroscopy,
chemiluminescence, mass spectrometry, and x-ray crystallography. We detected auto-S-
nitrosylation during enzymatic turnover by using chemiluminescence. Selective S …
(NO) as a potent mediator of cellular cytotoxicity or signaling. Yet, how this nitrosative stress
affects iNOS function in vivo is poorly understood. Here we define two specific non-heme
iNOS nitrosation sites discovered by combining UV-visible spectroscopy,
chemiluminescence, mass spectrometry, and x-ray crystallography. We detected auto-S-
nitrosylation during enzymatic turnover by using chemiluminescence. Selective S …