[HTML][HTML] Control of the DnaK chaperone cycle by substoichiometric concentrations of the co-chaperones DnaJ and GrpE
EV Pierpaoli, E Sandmeier, HJ Schönfeld… - Journal of Biological …, 1998 - ASBMB
The polypeptide binding and release cycle of the molecular chaperone DnaK (Hsp70) of
Escherichia coli is regulated by the two co-chaperones DnaJ and GrpE. Here, we show that
the DnaJ-triggered conversion of DnaK· ATP (T state) to DnaK· ADP· P i (R state), as
monitored by intrinsic protein fluorescence, is monophasic and occurs simultaneously with
ATP hydrolysis. This is in contrast with the T→ R conversion in the absence of DnaJ which is
biphasic, the first phase occurring simultaneously with the hydrolysis of ATP (Theyssen, H …
Escherichia coli is regulated by the two co-chaperones DnaJ and GrpE. Here, we show that
the DnaJ-triggered conversion of DnaK· ATP (T state) to DnaK· ADP· P i (R state), as
monitored by intrinsic protein fluorescence, is monophasic and occurs simultaneously with
ATP hydrolysis. This is in contrast with the T→ R conversion in the absence of DnaJ which is
biphasic, the first phase occurring simultaneously with the hydrolysis of ATP (Theyssen, H …