[PDF][PDF] Dissecting polyclonal vaccine-induced humoral immunity against HIV using systems serology

AW Chung, MP Kumar, KB Arnold, WH Yu, MK Schoen… - Cell, 2015 - cell.com
AW Chung, MP Kumar, KB Arnold, WH Yu, MK Schoen, LJ Dunphy, TJ Suscovich, N Frahm…
Cell, 2015cell.com
While antibody titers and neutralization are considered the gold standard for the selection of
successful vaccines, these parameters are often inadequate predictors of protective
immunity. As antibodies mediate an array of extra-neutralizing Fc functions, when
neutralization fails to predict protection, investigating Fc-mediated activity may help identify
immunological correlates and mechanism (s) of humoral protection. Here, we used an
integrative approach termed Systems Serology to analyze relationships among humoral …
Summary
While antibody titers and neutralization are considered the gold standard for the selection of successful vaccines, these parameters are often inadequate predictors of protective immunity. As antibodies mediate an array of extra-neutralizing Fc functions, when neutralization fails to predict protection, investigating Fc-mediated activity may help identify immunological correlates and mechanism(s) of humoral protection. Here, we used an integrative approach termed Systems Serology to analyze relationships among humoral responses elicited in four HIV vaccine trials. Each vaccine regimen induced a unique humoral "Fc fingerprint." Moreover, analysis of case:control data from the first moderately protective HIV vaccine trial, RV144, pointed to mechanistic insights into immune complex composition that may underlie protective immunity to HIV. Thus, multi-dimensional relational comparisons of vaccine humoral fingerprints offer a unique approach for the evaluation and design of novel vaccines against pathogens for which correlates of protection remain elusive.
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