Effects of cellular activation on anti-HIV-1 restriction factor expression profile in primary cells

RAS Raposo, M Abdel-Mohsen, M Bilska… - Journal of …, 2013 - Am Soc Microbiol
RAS Raposo, M Abdel-Mohsen, M Bilska, DC Montefiori, DF Nixon, SK Pillai
Journal of virology, 2013Am Soc Microbiol
Expression of cell-intrinsic antiviral factors suppresses HIV-1 replication. We hypothesized
that cellular activation modulates host restriction and susceptibility to HIV-1 infection. We
measured the gene expression of 34 antiviral factors in healthy peripheral blood
mononuclear cells (PBMC). Cellular activation induced expression of interferon-stimulated
gene 15 (ISG15), tripartite motif 5α (TRIM5α), bone marrow stromal cell antigen 2 (BST-
2)/tetherin, and certain apolipoprotein B mRNA editing enzyme 3 (APOBEC3) family …
Abstract
Expression of cell-intrinsic antiviral factors suppresses HIV-1 replication. We hypothesized that cellular activation modulates host restriction and susceptibility to HIV-1 infection. We measured the gene expression of 34 antiviral factors in healthy peripheral blood mononuclear cells (PBMC). Cellular activation induced expression of interferon-stimulated gene 15 (ISG15), tripartite motif 5α (TRIM5α), bone marrow stromal cell antigen 2 (BST-2)/tetherin, and certain apolipoprotein B mRNA editing enzyme 3 (APOBEC3) family members. Expression of RTF1, RNA polymerase II-associated factor 1 (PAF1), TRIM11, TRIM26, and BST-2/tetherin correlated with decreased HIV-1 infectivity. This report demonstrates synchronous effects of activation-induced antiviral genes on HIV-1 infectivity, providing candidates for pharmacological manipulation.
American Society for Microbiology