Staining of HLA-DR, Iba1 and CD68 in human microglia reveals partially overlapping expression depending on cellular morphology and pathology
DAE Hendrickx, CG van Eden, KG Schuurman… - Journal of …, 2017 - Elsevier
Abstract HLA-DR, Iba1 and CD68 are widely used microglia markers in human tissue.
However, due to differences in gene regulation, they may identify different activation stages
of microglia. Here, we directly compared the expression of HLA-DR, Iba1 and CD68 in
microglia with different phenotypes, ranging from ramified to amoeboid, to foamy
phagocytizing macrophages, in adjacent sections immunocytochemically double stained for
two of the markers. Material was used from patients diagnosed with multiple sclerosis (MS) …
However, due to differences in gene regulation, they may identify different activation stages
of microglia. Here, we directly compared the expression of HLA-DR, Iba1 and CD68 in
microglia with different phenotypes, ranging from ramified to amoeboid, to foamy
phagocytizing macrophages, in adjacent sections immunocytochemically double stained for
two of the markers. Material was used from patients diagnosed with multiple sclerosis (MS) …