[HTML][HTML] Increased frequency and clinical significance of myeloid-derived suppressor cells in human colorectal carcinoma

HL Sun, X Zhou, YF Xue, K Wang, YF Shen… - World journal of …, 2012 - ncbi.nlm.nih.gov
HL Sun, X Zhou, YF Xue, K Wang, YF Shen, JJ Mao, HF Guo, ZN Miao
World journal of gastroenterology: WJG, 2012ncbi.nlm.nih.gov
AIM: To investigate the frequency and clinical significance of the myeloid-derived
suppressor cells (MDSC) in human colorectal carcinoma (CRC). METHODS: Samples of
peripheral blood and tumor tissue from 49 CRC patients were analyzed. Mononuclear cells
were isolated by Ficoll-Hypaque density gradient centrifugation and were subjected to a flow
cytometry-based immunophenotypic analysis. RESULTS: A considerable increase in the
percentage of CD33+ HLA-DR-MDSCs was observed in the peripheral blood …
Abstract
AIM: To investigate the frequency and clinical significance of the myeloid-derived suppressor cells (MDSC) in human colorectal carcinoma (CRC).
METHODS: Samples of peripheral blood and tumor tissue from 49 CRC patients were analyzed. Mononuclear cells were isolated by Ficoll-Hypaque density gradient centrifugation and were subjected to a flow cytometry-based immunophenotypic analysis.
RESULTS: A considerable increase in the percentage of CD33+ HLA-DR-MDSCs was observed in the peripheral blood (1.89%±0.75%) and tumor tissues (2.99%±1.29%) of CRC patients as compared with that in the peripheral blood of healthy controls (0.54%±0.35%). This expanded CD33+ HLA-DR-subset exhibited immature myeloid cell markers, but not lineage markers, and showed up-regulation of CD18/CD11b expression as compared with the MDSCs from healthy donors. Further studies showed that the MDSC proportion in CRC peripheral blood was correlated with nodal metastasis
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