An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways

T Inoue, WD Heo, JS Grimley, TJ Wandless, T Meyer - Nature methods, 2005 - nature.com
T Inoue, WD Heo, JS Grimley, TJ Wandless, T Meyer
Nature methods, 2005nature.com
We made substantial advances in the implementation of a rapamycin-triggered
heterodimerization strategy. Using molecular engineering of different targeting and
enzymatic fusion constructs and a new rapamycin analog, Rho GTPases were directly
activated or inactivated on a timescale of seconds, which was followed by pronounced cell
morphological changes. As signaling processes often occur within minutes, such rapid
perturbations provide a powerful tool to investigate the role, selectivity and timing of Rho …
Abstract
We made substantial advances in the implementation of a rapamycin-triggered heterodimerization strategy. Using molecular engineering of different targeting and enzymatic fusion constructs and a new rapamycin analog, Rho GTPases were directly activated or inactivated on a timescale of seconds, which was followed by pronounced cell morphological changes. As signaling processes often occur within minutes, such rapid perturbations provide a powerful tool to investigate the role, selectivity and timing of Rho GTPase–mediated signaling processes.
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