Accurate profiling of lipidomes relies upon the quantitative and unbiased recovery of lipid
species from analyzed cells, fluids, or tissues and is usually achieved by two-phase
extraction with chloroform. We demonstrated that methyl-tert-butyl ether (MTBE) extraction
allows faster and cleaner lipid recovery and is well suited for automated shotgun profiling.
Because of MTBE's low density, lipid-containing organic phase forms the upper layer during
phase separation, which simplifies its collection and minimizes dripping losses …