Effects of Insert Size on Transposition Efficiency of the Sleeping Beauty Transposon in Mouse Cells

A Karsi, B Moav, P Hackett, Z Liu - Marine biotechnology, 2001 - Springer
Marine biotechnology, 2001Springer
Transposon vectors are widely used in prokaryotic and lower eukaryotic systems. However,
they were not available for use in vertebrate animals until the recent reconstitution of a
synthetic fish transposon, Sleeping Beauty (SB). The reacquisition of transposability of the
SB transposase fostered great enthusiasm for using transposon vectors as tools in
vertebrate animals, particularly for gene transfer to facilitate accelerated integration of
transgenes into chromosomes. Here, we report the effects of insert sizes on transposition …
Abstract
Transposon vectors are widely used in prokaryotic and lower eukaryotic systems. However, they were not available for use in vertebrate animals until the recent reconstitution of a synthetic fish transposon, Sleeping Beauty (SB). The reacquisition of transposability of the SB transposase fostered great enthusiasm for using transposon vectors as tools in vertebrate animals, particularly for gene transfer to facilitate accelerated integration of transgenes into chromosomes. Here, we report the effects of insert sizes on transposition efficiency of SB. A significant effect of insert size on efficiency of transposition by SB was found. The SB transposase enhanced the integration efficiency effectively for SB transposon up to approximately 5.6 kb, but lost its ability to enhance the integration efficiency when the transposon size was increased to 9.1 kb. This result indicates that the SB transposon system is highly applicable for transferring small genes, but may not be applicable for transferring very large genes.
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