Analysis of Clathrin-Mediated Endocytosis of Epidermal Growth Factor Receptor by RNA Interference*[Boxs]
F Huang, A Khvorova, W Marshall, A Sorkin - Journal of Biological Chemistry, 2004 - jbc.org
To identify proteins that participate in clathrin-mediated endocytosis of the epidermal growth
factor receptor (EGFR), 13 endocytic proteins were depleted in HeLa cells using highly
efficient small interfering RNAs that were designed using a novel selection algorithm. The
effects of small interfering RNAs on the ligand-induced endocytosis of EGFR were compared
with those effects on the constitutive internalization of the transferrin receptor. The knock-
downs of clathrin heavy chain and dynamin produced maximal inhibitory effects on the …
factor receptor (EGFR), 13 endocytic proteins were depleted in HeLa cells using highly
efficient small interfering RNAs that were designed using a novel selection algorithm. The
effects of small interfering RNAs on the ligand-induced endocytosis of EGFR were compared
with those effects on the constitutive internalization of the transferrin receptor. The knock-
downs of clathrin heavy chain and dynamin produced maximal inhibitory effects on the …