In situ hybridization to identify gut stem cells
A Gregorieff, H Clevers - Current protocols in stem cell biology, 2010 - Wiley Online Library
A Gregorieff, H Clevers
Current protocols in stem cell biology, 2010•Wiley Online LibraryIn recent years, considerable effort has been directed towards identifying the repertoire of
genes specifically expressed in adult stem cells. In this unit, we describe an in situ
hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa.
This methodology allows researchers to quickly visualize the expression profile of putative
stem cell markers with a high degree of sensitivity and resolution. Curr. Protoc. Stem Cell
Biol. 12: 2F. 1.1‐2F. 1.11.© 2010 by John Wiley & Sons, Inc.
genes specifically expressed in adult stem cells. In this unit, we describe an in situ
hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa.
This methodology allows researchers to quickly visualize the expression profile of putative
stem cell markers with a high degree of sensitivity and resolution. Curr. Protoc. Stem Cell
Biol. 12: 2F. 1.1‐2F. 1.11.© 2010 by John Wiley & Sons, Inc.
Abstract
In recent years, considerable effort has been directed towards identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution. Curr. Protoc. Stem Cell Biol. 12:2F.1.1‐2F.1.11. © 2010 by John Wiley & Sons, Inc.
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