A novel high-throughput method for accurate, rapid, and economical measurement of multiple type 1 diabetes autoantibodies
W Woo, JM LaGasse, Z Zhou, R Patel… - Journal of …, 2000 - Elsevier
Prediction of Type 1 diabetes for study of preventive therapies requires screening the
general population, where 85% of new cases occur. Even with HLA-based prescreening,
nearly 20% of all children will need multiple serum autoantibody testings. High-throughput,
economical, and accurate methods are therefore essential. We have developed such a
radiobinding method, using 96-well microtiter plates and a novel immune complex capture
method via membrane-bound Protein A. Each microtiter plate contained a standard negative …
general population, where 85% of new cases occur. Even with HLA-based prescreening,
nearly 20% of all children will need multiple serum autoantibody testings. High-throughput,
economical, and accurate methods are therefore essential. We have developed such a
radiobinding method, using 96-well microtiter plates and a novel immune complex capture
method via membrane-bound Protein A. Each microtiter plate contained a standard negative …