[HTML][HTML] Use of high performance liquid chromatography-electrospray ionization-tandem mass spectrometry for the analysis of ceramide-1-phosphate levels [S]
DS Wijesinghe, JC Allegood, LB Gentile, TE Fox… - Journal of lipid …, 2010 - ASBMB
Ceramide-1-phosphate (C1P) is a bioactive sphingolipid with roles in several biological
processes. Currently, high performance liquid chromatography-electrospray ionization-
tandem mass spectrometry (HPLC ESI-MS/MS) offers the most efficient method of
quantifying C1P. However, the published protocols have several drawbacks causing
overestimations and carryovers. Here, the reported overestimation of C1P was shown to be
due to incomplete neutralization of base hydrolyzed lipid extracts leading to the hydrolysis of …
processes. Currently, high performance liquid chromatography-electrospray ionization-
tandem mass spectrometry (HPLC ESI-MS/MS) offers the most efficient method of
quantifying C1P. However, the published protocols have several drawbacks causing
overestimations and carryovers. Here, the reported overestimation of C1P was shown to be
due to incomplete neutralization of base hydrolyzed lipid extracts leading to the hydrolysis of …