[HTML][HTML] Quantitative analysis of sphingolipids for lipidomics using triple quadrupole and quadrupole linear ion trap mass spectrometers [S]
RL Shaner, JC Allegood, H Park, E Wang, S Kelly… - Journal of lipid …, 2009 - ASBMB
Sphingolipids are a highly diverse category of bioactive compounds. This article describes
methods that have been validated for the extraction, liquid chromatographic (LC) separation,
identification and quantitation of sphingolipids by electrospray ionization, tandem mass
spectrometry (ESI-MS/MS) using triple quadrupole (QQQ, API 3000) and quadrupole-linear-
ion trap (API 4000 QTrap, operating in QQQ mode) mass spectrometers. Advantages of the
QTrap included: greater sensitivity, similar ionization efficiencies for sphingolipids with …
methods that have been validated for the extraction, liquid chromatographic (LC) separation,
identification and quantitation of sphingolipids by electrospray ionization, tandem mass
spectrometry (ESI-MS/MS) using triple quadrupole (QQQ, API 3000) and quadrupole-linear-
ion trap (API 4000 QTrap, operating in QQQ mode) mass spectrometers. Advantages of the
QTrap included: greater sensitivity, similar ionization efficiencies for sphingolipids with …
