[HTML][HTML] Fully human monoclonal antibodies from antibody secreting cells after vaccination with Pneumovax® 23 are serotype specific and facilitate …
K Smith, JJ Muther, AL Duke, E McKee, NY Zheng… - Immunobiology, 2013 - Elsevier
B lymphocyte memory generates antibody-secreting cells (ASCs) that represent a source of
protective antibodies that may be exploited for therapeutics. Here we vaccinated four donors
with Pneumovax® 23 and produced human monoclonal antibodies (hmAbs) from ASCs. We
have cloned 137 hmAbs and the specificities of these antibodies encompass 19 of the 23
serotypes in the vaccine, as well as cell wall polysaccharide (CWPS). Although the majority
of the antibodies are serotype specific, 12% cross-react with two serotypes. The …
protective antibodies that may be exploited for therapeutics. Here we vaccinated four donors
with Pneumovax® 23 and produced human monoclonal antibodies (hmAbs) from ASCs. We
have cloned 137 hmAbs and the specificities of these antibodies encompass 19 of the 23
serotypes in the vaccine, as well as cell wall polysaccharide (CWPS). Although the majority
of the antibodies are serotype specific, 12% cross-react with two serotypes. The …