A unifying paradigm for transcriptional heterogeneity and squamous features in pancreatic ductal adenocarcinoma

A Hayashi, J Fan, R Chen, Y Ho, AP Makohon-Moore… - Nature Cancer, 2020 - nature.com
A Hayashi, J Fan, R Chen, Y Ho, AP Makohon-Moore, N Lecomte, Y Zhong, J Hong
Nature Cancer, 2020nature.com
Pancreatic cancer expression profiles largely reflect a classical or basal-like phenotype. The
extent to which these profiles vary within a patient is unknown. We integrated evolutionary
analysis and expression profiling in multiregion-sampled metastatic pancreatic cancers,
finding that squamous features are the histologic correlate of an RNA-seq-defined basal-like
subtype. In patients with coexisting basal and squamous and classical and glandular
morphology, phylogenetic studies revealed that squamous morphology represented a …
Abstract
Pancreatic cancer expression profiles largely reflect a classical or basal-like phenotype. The extent to which these profiles vary within a patient is unknown. We integrated evolutionary analysis and expression profiling in multiregion-sampled metastatic pancreatic cancers, finding that squamous features are the histologic correlate of an RNA-seq-defined basal-like subtype. In patients with coexisting basal and squamous and classical and glandular morphology, phylogenetic studies revealed that squamous morphology represented a subclonal population in an otherwise classical and glandular tumor. Cancers with squamous features were significantly more likely to have clonal mutations in chromatin modifiers, intercellular heterogeneity for MYC amplification and entosis. These data provide a unifying paradigm for integrating basal-type expression profiles, squamous histology and somatic mutations in chromatin modifier genes in the context of clonal evolution of pancreatic cancer.
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