Noninvasive methods to diagnose rejection of renal allografts are unavailable. Mass
spectrometry followed by multiple-reaction monitoring provides a unique approach to
identify disease-specific urine peptide biomarkers. Here, we performed urine peptidomic
analysis of 70 unique samples from 50 renal transplant patients and 20 controls (n= 20),
identifying a specific panel of 40 peptides for acute rejection (AR). Peptide sequencing
revealed suggestive mechanisms of graft injury with roles for proteolytic degradation of …