Targeted deletion of nuclear factor κB p50 enhances cardiac remodeling and dysfunction following myocardial infarction

L Timmers, JK van Keulen, IE Hoefer… - Circulation …, 2009 - Am Heart Assoc
L Timmers, JK van Keulen, IE Hoefer, MFL Meijs, B van Middelaar, K den Ouden…
Circulation research, 2009Am Heart Assoc
Myocardial infarction is commonly complicated by left ventricular remodeling, a process that
leads to cardiac dilatation, congestive heart failure and death. The innate immune system
plays a pivotal role in the remodeling process via nuclear factor (NF)-κB activation. The NF-
κB transcription factor family includes several subunits (p50, p52, p65, c-Rel, and Rel B) that
respond to myocardial ischemia. The function of NF-κB p50, however, is controversial in this
process. To clarify the role of NF-κB p50 in postinfarct left ventricular remodeling, myocardial …
Myocardial infarction is commonly complicated by left ventricular remodeling, a process that leads to cardiac dilatation, congestive heart failure and death. The innate immune system plays a pivotal role in the remodeling process via nuclear factor (NF)-κB activation. The NF-κB transcription factor family includes several subunits (p50, p52, p65, c-Rel, and Rel B) that respond to myocardial ischemia. The function of NF-κB p50, however, is controversial in this process. To clarify the role of NF-κB p50 in postinfarct left ventricular remodeling, myocardial infarction was induced in wild-type 129Bl6 mice and NF-κB p50-deficient mice. Without affecting infarct size, deletion of NF-κB p50 markedly increased the extent of expansive remodeling (end-diastolic volume: 176±13 μL versus 107±11 μL; P=0.003) and aggravated systolic dysfunction (left ventricular ejection fraction: 16.1±1.5% versus 24.7±3.7%; P=0.029) in a 28-day time period. Interstitial fibrosis and hypertrophy in the noninfarcted myocardium was increased in NF-κB p50 knockout mice. In the infarct area, a lower collagen density was observed, which was accompanied by an increased number of macrophages, higher gelatinase activity and increased inflammatory cytokine expression. In conclusion, targeted deletion of NF-κB p50 results in enhanced cardiac remodeling and functional deterioration following myocardial infarction by increasing matrix remodeling and inflammation.
Am Heart Assoc