Quantification of total and 2-LTR (long terminal repeat) HIV DNA, HIV RNA and herpesvirus DNA in PBMCs
Almost all individuals infected with human immunodeficiency virus (HIV) are also infected
with cytomegalovirus (CMV) and Epstein Barr virus (EBV). The aims of our studies have
included characterizing and measuring the latent HIV reservoir and understanding the
association between asymptomatic replication of CMV (and other herpesvirus, including
EBV) and this HIV reservoir (Gianella et al., 2014). This protocol was designed to
simultaneously co-extract DNA and RNA from the same peripheral blood mononuclear cell …
with cytomegalovirus (CMV) and Epstein Barr virus (EBV). The aims of our studies have
included characterizing and measuring the latent HIV reservoir and understanding the
association between asymptomatic replication of CMV (and other herpesvirus, including
EBV) and this HIV reservoir (Gianella et al., 2014). This protocol was designed to
simultaneously co-extract DNA and RNA from the same peripheral blood mononuclear cell …