JAK/STAT1 signaling promotes HMGB1 hyperacetylation and nuclear translocation

B Lu, DJ Antoine, K Kwan… - Proceedings of the …, 2014 - National Acad Sciences
B Lu, DJ Antoine, K Kwan, P Lundbäck, H Wähämaa, H Schierbeck, M Robinson…
Proceedings of the National Academy of Sciences, 2014National Acad Sciences
Extracellular high-mobility group box (HMGB) 1 mediates inflammation during sterile and
infectious injury and contributes importantly to disease pathogenesis. The first critical step in
the release of HMGB1 from activated immune cells is mobilization from the nucleus to the
cytoplasm, a process dependent upon hyperacetylation within two HMGB1 nuclear
localization sequence (NLS) sites. The inflammasomes mediate the release of cytoplasmic
HMGB1 in activated immune cells, but the mechanism of HMGB1 translocation from nucleus …
Extracellular high-mobility group box (HMGB)1 mediates inflammation during sterile and infectious injury and contributes importantly to disease pathogenesis. The first critical step in the release of HMGB1 from activated immune cells is mobilization from the nucleus to the cytoplasm, a process dependent upon hyperacetylation within two HMGB1 nuclear localization sequence (NLS) sites. The inflammasomes mediate the release of cytoplasmic HMGB1 in activated immune cells, but the mechanism of HMGB1 translocation from nucleus to cytoplasm was previously unknown. Here, we show that pharmacological inhibition of JAK/STAT1 inhibits LPS-induced HMGB1 nuclear translocation. Conversely, activation of JAK/STAT1 by type 1 interferon (IFN) stimulation induces HMGB1 translocation from nucleus to cytoplasm. Mass spectrometric analysis unequivocally revealed that pharmacological inhibition of the JAK/STAT1 pathway or genetic deletion of STAT1 abrogated LPS- or type 1 IFN-induced HMGB1 acetylation within the NLS sites. Together, these results identify a critical role of the JAK/STAT1 pathway in mediating HMGB1 cytoplasmic accumulation for subsequent release, suggesting that the JAK/STAT1 pathway is a potential drug target for inhibiting HMGB1 release.
National Acad Sciences