Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production
MC Glaum, S Narula, D Song, Y Zheng… - Journal of allergy and …, 2009 - Elsevier
BACKGROUND: Toll-like receptors contribute to the establishment of adaptive immune
responses. OBJECTIVE: The reported studies were conducted to examine the effects of the
Toll-like receptor (TLR)–7 ligand, resiquimod, on human naive B-cell differentiation.
METHODS: Naive human B cells were cultured with resiquimod in the presence or absence
of IL-2 and IL-10. Secreted IgM and IgG were measured by ELISA, and IL-6, IL-10, and IFN-α
were measured by a multiplex protein array. Cell proliferation was assessed by measuring …
responses. OBJECTIVE: The reported studies were conducted to examine the effects of the
Toll-like receptor (TLR)–7 ligand, resiquimod, on human naive B-cell differentiation.
METHODS: Naive human B cells were cultured with resiquimod in the presence or absence
of IL-2 and IL-10. Secreted IgM and IgG were measured by ELISA, and IL-6, IL-10, and IFN-α
were measured by a multiplex protein array. Cell proliferation was assessed by measuring …