High-throughput isolation of immunoglobulin genes from single human B cells and expression as monoclonal antibodies
HX Liao, MC Levesque, A Nagel, A Dixon… - Journal of virological …, 2009 - Elsevier
Defining human B cell repertoires to viral pathogens is critical for design of vaccines that
induce broadly protective antibodies to infections such as HIV-1 and influenza. Single B cell
sorting and cloning of immunoglobulin (Ig) heavy-and light-chain variable regions (VH and
VL) is a powerful technology for defining anti-viral B cell repertoires. However, the Ig-cloning
step is time-consuming and prevents high-throughput analysis of the B cell repertoire. Novel
linear Ig heavy-and light-chain gene expression cassettes were designed to express Ig VH …
induce broadly protective antibodies to infections such as HIV-1 and influenza. Single B cell
sorting and cloning of immunoglobulin (Ig) heavy-and light-chain variable regions (VH and
VL) is a powerful technology for defining anti-viral B cell repertoires. However, the Ig-cloning
step is time-consuming and prevents high-throughput analysis of the B cell repertoire. Novel
linear Ig heavy-and light-chain gene expression cassettes were designed to express Ig VH …