Isoprenoid lipid metabolism in the retina: dynamics of squalene and cholesterol incorporation and turnover in frog rod outer segment membranes
SJ Fliesler, R Florman, RK Keller - Experimental eye research, 1995 - Elsevier
Frogs were injected intravitreally with [3H] acetate, and the formation of [3H]-labeled
squalene and cholesterol in the retina and their incorporation into rod outer segment (ROS)
membranes were evaluated biochemically over a 60-day time course. ROS [3H] squalene
specific activity was maximal by 1–3 days, then declined with a half-time of∼ 20–30 days. In
contrast, the specific activity of ROS [3H] cholesterol initially increased to a level
substantially less than that of [3H] squalene, and then remained constant. Thus, ROS …
squalene and cholesterol in the retina and their incorporation into rod outer segment (ROS)
membranes were evaluated biochemically over a 60-day time course. ROS [3H] squalene
specific activity was maximal by 1–3 days, then declined with a half-time of∼ 20–30 days. In
contrast, the specific activity of ROS [3H] cholesterol initially increased to a level
substantially less than that of [3H] squalene, and then remained constant. Thus, ROS …