[HTML][HTML] Novel synovial fluid recovery method allows for quantification of a marker of arthritis in mice

DR Seifer, BD Furman, F Guilak, SA Olson… - Osteoarthritis and …, 2008 - Elsevier
DR Seifer, BD Furman, F Guilak, SA Olson, SC Brooks III, VB Kraus
Osteoarthritis and cartilage, 2008Elsevier
OBJECTIVE: We evaluated three methodologies–a calcium sodium alginate compound
(CSAC), polyacrylate beads (PABs), and Whatman paper recovery (WPR)–for the ability to
recover synovial fluid (SF) from mouse knees in a manner that facilitated biochemical marker
analysis. METHODS: Pilot testing of each of these recovery vehicles was conducted using
small volumes of waste human SF. CSAC emerged as the method of choice, and was used
to recover and quantify SF from the knees of C57BL/6 mice (n= 12), six of which were given …
OBJECTIVE
We evaluated three methodologies – a calcium sodium alginate compound (CSAC), polyacrylate beads (PABs), and Whatman paper recovery (WPR) – for the ability to recover synovial fluid (SF) from mouse knees in a manner that facilitated biochemical marker analysis.
METHODS
Pilot testing of each of these recovery vehicles was conducted using small volumes of waste human SF. CSAC emerged as the method of choice, and was used to recover and quantify SF from the knees of C57BL/6 mice (n=12), six of which were given left knee articular fractures. SF concentrations of cartilage oligomeric matrix protein (COMP) were measured by enzyme-linked immunosorbent assay.
RESULTS
The mean concentration ratio [(COMPleft knee)/(COMPright knee)] was higher in the mice subjected to articular fracture when compared to the non-fracture mice (P=0.026). The mean total COMP ratio (taking into account the quantitative recovery of SF) best discriminated between fracture and non-fracture knees (P=0.004).
CONCLUSIONS
Our results provide the first direct evidence of accelerated joint tissue turnover in a mouse model responding to acute joint injury. These data strongly suggest that mouse SF recovery is feasible and that biomarker analysis of collected SF samples can augment traditional histological analyses in mouse models of arthritis.
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