Overlapping elements in the guanylate-binding protein gene promoter mediate transcriptional induction by alpha and gamma interferons
The gene encoding a 67-kDa cytoplasmic guanylate-binding protein (GBP) is
transcriptionally induced in cells exposed to interferon of either type I (alpha interferon [IFN-
α] or INF-β) or type II (IFN-γ). The promoter of the GBP gene was cloned and found to contain
an IFN-α-stimulated response element, which mediated the response of the GBP gene to
IFN-α. On the basis of transfection experiments with recombinant plasmids, two different
elements were delineated. Both were required to obtain the maximal response of the GBP …
transcriptionally induced in cells exposed to interferon of either type I (alpha interferon [IFN-
α] or INF-β) or type II (IFN-γ). The promoter of the GBP gene was cloned and found to contain
an IFN-α-stimulated response element, which mediated the response of the GBP gene to
IFN-α. On the basis of transfection experiments with recombinant plasmids, two different
elements were delineated. Both were required to obtain the maximal response of the GBP …
Abstract
The gene encoding a 67-kDa cytoplasmic guanylate-binding protein (GBP) is transcriptionally induced in cells exposed to interferon of either type I (alpha interferon [IFN-α] or INF-β) or type II (IFN-γ). The promoter of the GBP gene was cloned and found to contain an IFN-α-stimulated response element, which mediated the response of the GBP gene to IFN-α. On the basis of transfection experiments with recombinant plasmids, two different elements were delineated. Both were required to obtain the maximal response of the GBP gene to IFN-γ: the IFN-α-stimulated response element and an overlapping element termed the IFN-γ activation site. Different proteins that act on each element were investigated, and their possible involvement in IFN-γ-induced transcriptional regulation is discussed.
American Society for Microbiology