CD27⁺ memory B cells are reduced in the blood of patients with chronic granulomatous disease (CGD) for reasons and consequences that remain unclear. Here we confirm not only decreased CD27⁺ but also IgG⁺ B cells in the blood of CGD patients compared with healthy donors (HDs). However, among IgG⁺ B cells, the ratio of CD27⁻ to CD27⁺ was significantly higher in CGD patients compared with HDs. Similar to conventional memory B cells, CD27⁻IgG⁺ B cells of CGD patients expressed activation markers and had undergone somatic hypermutation, albeit at levels lower than their CD27⁺ counterparts. Functional analyses revealed slight reductions in frequencies of total IgG but not influenza-specific memory B-cell responses, as measured by Elispot in CGD patients compared with HDs. Serum IgG levels and influenza-specific antibodies were also normal in these CGD patients. Finally, we provide evidence that influenza-specific memory B cells can be present within the CD27⁻IgG⁺ B-cell compartment. Together, these findings show that, despite reduced circulating CD27⁺ memory B cells, CGD patients maintain an intact humoral immunologic memory, with potential contribution from CD27⁻ B cells.