Deep sequencing and Circos analyses of antibody libraries reveal antigen-driven selection of Ig VH genes during HIV-1 infection
M Xiao, P Prabakaran, W Chen, B Kessing… - … and molecular pathology, 2013 - Elsevier
The vast diversity of antibody repertoires is largely attributed to heavy chain (VH)
recombination of variable (V), diversity (D) and joining (J) gene segments. We used 454
sequencing information of the variable domains of the antibody heavy chain repertoires from
neonates, normal adults and an HIV-1-infected individual, to analyze, with Circos software,
the VDJ pairing patterns at birth, adulthood and a time-dependent response to HIV-1
infection. Our comparative analyses of the Ig VDJ repertoires from these libraries indicated …
recombination of variable (V), diversity (D) and joining (J) gene segments. We used 454
sequencing information of the variable domains of the antibody heavy chain repertoires from
neonates, normal adults and an HIV-1-infected individual, to analyze, with Circos software,
the VDJ pairing patterns at birth, adulthood and a time-dependent response to HIV-1
infection. Our comparative analyses of the Ig VDJ repertoires from these libraries indicated …