Measurement of glycosaminoglycan release from cartilage explants

JS Mort, PJ Roughley - Arthritis research: methods and protocols volume 1, 2007 - Springer
JS Mort, PJ Roughley
Arthritis research: methods and protocols volume 1, 2007Springer
Quantitation of glycosaminoglycans (GAGs) in the form of aggrecan fragments released from
cartilage in culture is a simple way to determine the efficacy of different cytokines alone or in
combination in simulating cartilage catabolism. Two approaches for GAG assay are
described, with special attention being paid to the advantages and limitations of each
method
Abstract
Quantitation of glycosaminoglycans (GAGs) in the form of aggrecan fragments released from cartilage in culture is a simple way to determine the efficacy of different cytokines alone or in combination in simulating cartilage catabolism. Two approaches for GAG assay are described, with special attention being paid to the advantages and limitations of each method
Springer