[HTML][HTML] Properties, production, and applications of camelid single-domain antibody fragments

MM Harmsen, HJ De Haard - Applied microbiology and biotechnology, 2007 - Springer
MM Harmsen, HJ De Haard
Applied microbiology and biotechnology, 2007Springer
Camelids produce functional antibodies devoid of light chains of which the single N-terminal
domain is fully capable of antigen binding. These single-domain antibody fragments (VHHs
or NanobodiesŪ) have several advantages for biotechnological applications. They are well
expressed in microorganisms and have a high stability and solubility. Furthermore, they are
well suited for construction of larger molecules and selection systems such as phage, yeast,
or ribosome display. This minireview offers an overview of (1) their properties as compared …
Abstract
Camelids produce functional antibodies devoid of light chains of which the single N-terminal domain is fully capable of antigen binding. These single-domain antibody fragments (VHHs or NanobodiesŪ) have several advantages for biotechnological applications. They are well expressed in microorganisms and have a high stability and solubility. Furthermore, they are well suited for construction of larger molecules and selection systems such as phage, yeast, or ribosome display. This minireview offers an overview of (1) their properties as compared to conventional antibodies, (2) their production in microorganisms, with a focus on yeasts, and (3) their therapeutic applications.
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