Chorioallantoic membrane assay: vascularized 3-dimensional cell culture system for human prostate cancer cells as an animal substitute model

K Kunzi-Rapp, F Genze, R KÜFER, E Reich… - The Journal of …, 2001 - Elsevier
PURPOSE: Chorioallantoic membranes have been used as a reliable biomedical assay
system for many years. Chicken eggs in the early phase of breeding are between in vitro
and in vivo systems but may provide an immunodeficient, vascularized test environment. We
tested this model as an in vivo system for prostate cancer research. MATERIALS AND
METHODS: Single cell suspensions of LNCaP, PC-3 and Tsu-Pr1 human prostatic cancer
cell lines as well as 2 immortalized normal human prostate epithelial cell lines were …