T cell fate and clonality inference from single-cell transcriptomes
Nature methods, 2016•nature.com
We developed TraCeR, a computational method to reconstruct full-length, paired T cell
receptor (TCR) sequences from T lymphocyte single-cell RNA sequence data. TraCeR links
T cell specificity with functional response by revealing clonal relationships between cells
alongside their transcriptional profiles. We found that T cell clonotypes in a mouse
Salmonella infection model span early activated CD4+ T cells as well as mature effector and
memory cells.
receptor (TCR) sequences from T lymphocyte single-cell RNA sequence data. TraCeR links
T cell specificity with functional response by revealing clonal relationships between cells
alongside their transcriptional profiles. We found that T cell clonotypes in a mouse
Salmonella infection model span early activated CD4+ T cells as well as mature effector and
memory cells.
Abstract
We developed TraCeR, a computational method to reconstruct full-length, paired T cell receptor (TCR) sequences from T lymphocyte single-cell RNA sequence data. TraCeR links T cell specificity with functional response by revealing clonal relationships between cells alongside their transcriptional profiles. We found that T cell clonotypes in a mouse Salmonella infection model span early activated CD4+ T cells as well as mature effector and memory cells.
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