[HTML][HTML] The human prepro-orexin gene regulatory region that activates gene expression in the lateral region and represses it in the medial regions of the …

T Moriguchi, T Sakurai, S Takahashi, K Goto… - Journal of Biological …, 2002 - ASBMB
Prepro-orexin is a precursor of the neuropeptides orexin-A and-B, which are localized in the
neuronal population of the lateral hypothalamic area (LHA). We wished to elucidate the
mechanisms by which the prepro-orexin gene is specifically activated in orexin neurons in
the LHA. The 3.2-kb 5′-flanking region of the human prepro-orexin gene is sufficient for the
specific expression of anEscherichia coli lacZ reporter gene in orexin neurons. Therefore,
we examined a series of reporter constructs harboring this 3.2-kb regulatory region or its …