Production and characterization of clinical grade Escherichia coli derived Plasmodium falciparum 42 kDa merozoite surface protein 1 (MSP142) in the absence of an …
RL Shimp Jr, LB Martin, Y Zhang, BS Henderson… - Protein expression and …, 2006 - Elsevier
The 42kDa cleavage product from the carboxyl end of the Plasmodium falciparum merozoite
surface protein 1 (MSP142) is an important blood-stage malaria vaccine target. Several
recombinant protein expression systems have been used for production of MSP142
including yeast (Saccharomyces cerevisiae and Pichia pastoris), Escherichia coli,
baculovirus and transgenic animals. To date, all of the reported recombinant proteins
include a 6× His affinity tag to facilitate purification, including three MSP142 clinical grade …
surface protein 1 (MSP142) is an important blood-stage malaria vaccine target. Several
recombinant protein expression systems have been used for production of MSP142
including yeast (Saccharomyces cerevisiae and Pichia pastoris), Escherichia coli,
baculovirus and transgenic animals. To date, all of the reported recombinant proteins
include a 6× His affinity tag to facilitate purification, including three MSP142 clinical grade …
