Systemic inflammation induces release of cell-free DNA from hematopoietic and parenchymal cells in mice and humans
AJ van der Meer, A Kroeze, AJ Hoogendijk… - Blood advances, 2019 - Elsevier
AJ van der Meer, A Kroeze, AJ Hoogendijk, AA Soussan, CE van der Schoot, WA Wuillemin…
Blood advances, 2019•ElsevierMethods Endotoxemia was induced in 8 healthy, nonsmoking Caucasian male volunteers
by an IV injection with Escherichia coli lipopolysaccharide (LPS; US standard reference
endotoxin, kindly provided by the National Institutes of Health, Bethesda, MD) at 4 ng/kg
body weight. Blood was obtained t=− 3, 0, 0.5, 1, 1.5, 2, 3, 4, 5, 6, 8, 12, and 21 hours
relative to LPS injection. Plasma samples of sepsis patients, described elsewhere, were
obtained at admission and thereafter daily until day 4. 17
by an IV injection with Escherichia coli lipopolysaccharide (LPS; US standard reference
endotoxin, kindly provided by the National Institutes of Health, Bethesda, MD) at 4 ng/kg
body weight. Blood was obtained t=− 3, 0, 0.5, 1, 1.5, 2, 3, 4, 5, 6, 8, 12, and 21 hours
relative to LPS injection. Plasma samples of sepsis patients, described elsewhere, were
obtained at admission and thereafter daily until day 4. 17
Methods
Endotoxemia was induced in 8 healthy, nonsmoking Caucasian male volunteers by an IV injection with Escherichia coli lipopolysaccharide (LPS; US standard reference endotoxin, kindly provided by the National Institutes of Health, Bethesda, MD) at 4 ng/kg body weight. Blood was obtained t=− 3, 0, 0.5, 1, 1.5, 2, 3, 4, 5, 6, 8, 12, and 21 hours relative to LPS injection. Plasma samples of sepsis patients, described elsewhere, were obtained at admission and thereafter daily until day 4. 17
Elsevier