Inverse correlation between the extent of N-glycan branching and intercellular adhesion in epithelia: contribution of the Na, K-ATPase β1 subunit
O Vagin, E Tokhtaeva, I Yakubov, E Shevchenko… - Journal of biological …, 2008 - ASBMB
The majority of cell adhesion molecules are N-glycosylated, but the role of N-glycans in
intercellular adhesion in epithelia remains ill-defined. Reducing N-glycan branching of
cellular glycoproteins by swainsonine, the inhibitor of N-glycan processing, tightens and
stabilizes cell-cell junctions as detected by a 3-fold decrease in the paracellular permeability
and a 2-3-fold increase in the resistance of the adherens junction proteins to extraction by
non-ionic detergent. In addition, exposure of cells to swainsonine inhibits motility of MDCK …
intercellular adhesion in epithelia remains ill-defined. Reducing N-glycan branching of
cellular glycoproteins by swainsonine, the inhibitor of N-glycan processing, tightens and
stabilizes cell-cell junctions as detected by a 3-fold decrease in the paracellular permeability
and a 2-3-fold increase in the resistance of the adherens junction proteins to extraction by
non-ionic detergent. In addition, exposure of cells to swainsonine inhibits motility of MDCK …
