Towards an HIV-1 cure: measuring the latent reservoir

KM Bruner, NN Hosmane, RF Siliciano - Trends in microbiology, 2015 - cell.com
Trends in microbiology, 2015cell.com
The latent reservoir (LR) of HIV-1 in resting memory CD4+ T cells serves as a major barrier
to curing HIV-1 infection. While many PCR-and culture-based assays have been used to
measure the size of the LR, correlation between results of different assays is poor and recent
studies indicate that no available assay provides an accurate measurement of reservoir size.
The discrepancies between assays are a hurdle to clinical trials that aim to measure the
efficacy of HIV-1 eradication strategies. Here we describe the advantages and …
The latent reservoir (LR) of HIV-1 in resting memory CD4+ T cells serves as a major barrier to curing HIV-1 infection. While many PCR- and culture-based assays have been used to measure the size of the LR, correlation between results of different assays is poor and recent studies indicate that no available assay provides an accurate measurement of reservoir size. The discrepancies between assays are a hurdle to clinical trials that aim to measure the efficacy of HIV-1 eradication strategies. Here we describe the advantages and disadvantages of various approaches to measuring the LR.
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